ABSTRACT
Introducción: la Cirugía Ambulatoria es un modelo organizativo de asistencia quirúrgica, que permite tratar a pacientes bien seleccionados en forma efectiva, segura y eficiente, sin necesidad de contar con una cama de internación. La Colecistectomía laparoscópica es considerada actualmente el tratamiento de elección de la litiasis vesicular sintomática. El uso de la laparoscopía reduce la estadía hospitalaria y el tiempo de recuperación postoperatoria. Objetivo: evaluar la factibilidad, seguridad y beneficios de la realización de Colecistectomías Laparoscópica en Pacientes en una Unidad de Cirugía Ambulatoria. Material y Métodos: el registro de los pacientes durante el peroperatorio de Colecistectomías Laparoscópicas desde Enero del 2012 a Diciembre del 2014, en la Unidad de Cirugía Ambulatoria del Policlínico del Docente. Resultados: Del total de las cirugías, 623 fueron Colecistectomías Laparoscópicas de las cuales 519 se realizaron en forma programadas e ingresaron por U.C.A. y 104 por guardia. El tiempo promedio de permanencia en el área es de 8,25 hs. El índice de ambulatorización es del 83.3%. Conclusión: La realización de la Colecistectomía Laparoscópica en una modalidad ambulatoria en pacientes adecuadamente seleccionados, constituye una opción interesante para aumentar el número de colecistectomías en nuestro medio y modifica positivamente la percepción del paciente sobre su enfermedad y acelera su recuperación.
Introduction: Ambulatory Surgery is a surgical care organizationalmodel that allows selected patients to be treated effectively, safely and efficiently, without taken a bed of admission. Laparoscopic cholecystectomy is now considered the Gold Standard treatmentfor symptomatic gallstones. The laparoscopy surgery reduceshospital stay and postoperative time recovery.Objective: To evaluate the feasibility, safety and benefits ofperforming laparoscopic cholecystectomy in patients in a DaySurgery Unit (D.S.U). Material and Methods: the registration of patients during theperioperative Laparoscopic cholecystectomy from January 2012to December 2014, in the Day Surgery Unit from the Policinico delDocente.Results: Of all the surgeries, Laparoscopic cholecystectomy were623 of which 519 were performed as scheduled and entered byD.S.U. and 104 were admitted at the Urgencys Appartment. The average time spent in the area is 8.25 pm. Ambulatory index is83.3%. Conclusion: The realization of laparoscopic cholecystectomy inan outpatient based in properly selected patients, is an interestingmodel to increase the number of cholecystectomies in ourenvironment and positivel y change the perception of the patientabout his illness and recovery accelerates option.
Subject(s)
Animals , Ambulatory Care , Urinary Bladder Calculi/diagnosis , Urinary Bladder Calculi/therapy , Cholecystectomy, Laparoscopic/methods , Cholecystectomy, LaparoscopicABSTRACT
Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragments using the PCR technique. The arrays were hybridized against cDNAs labeled during reverse transcription reactions and which were obtained from bacteria grown under two different conditions (liquid XDM2 and liquid BCYE). All data were statistically analyzed to verify which genes were differentially expressed. In addition to exploring conditions for X. fastidiosa genome-wide transcriptome analysis, the present work observed the differential expression of several classes of genes (energy, protein, amino acid and nucleotide metabolism, transport, degradation of substances, toxins and hypothetical proteins, among others). The understanding of expressed genes in these two different media will be useful in comprehending the metabolic characteristics of X. fastidiosa, and in evaluating how important certain genes are for the functioning and survival of these bacteria in plants.